Tissue-resident memory T cells (TRM) are non-recirculating cells that reside in lymphoid and non-lymphoid organs, where they provide local protection against infection and cancer. These cells have also been implicated in mediating pathological responses in autoimmune diseases, where dislodging these cells from the tissue represents an attractive therapeutic intervention. It has recently been shown that skin TRM utilize exogenous fatty acids for their long-term survival, in contrast to circulating memory T cells that rely upon endogenously synthetized fatty acids and oxidative phosphorylation. However, it is not clear whether this is true of TRM in other tissues, nor whether FABPs expression are involved in their development or maintenance. Here, we show that TRM display unique expression profiles of fatty acid binding proteins (FABPs) in cells isolated from different organs. Expression of FABPs on other tissue-resident leukocytes mirrors the differential FABP expression observed on TRM within the same microenvironment. FABP expression is upregulated subsequent to tissue entry and increases over time, along with acquisition of the tissue-resident phenotype. Moreover, we show that FABP expression is dynamically modified by the local microenvironment, allowing TRM progeny to adapt to new tissues upon redistribution. Our study demonstrates tissue-specific adaptation of FABP expression, implicating a role in the regulation of TRM populations in different organs.