The Candida albicans cell wall is an essential cellular structure which provides protection from physical and chemical insults, and is involved in immune-recognition and immune-avoidance. Chitin and β(1,3)-glucan are the main structural components and are thought to form the inner layers of the cell wall, with highly mannosylated cell wall proteins attached to these sugars through various linkages. Cartoons representing our best guess of the structure of the cell wall have been published, but few have directly tested their nature. We have examined the precise structure, location and molecular sizes of the cell wall components using transmission electron microscopy and tomography, and have tested predictions of the cell wall models using mutants and agents that perturb the normal cell wall structure. We present the first 3D model and to-scale cartoon of the C. albicans cell wall which impact our understanding of the interactions of this fungal pathogen with its host.